The Octave^™ Chromatography System:
Prep LC Re-imagined...

The Octave Chromatography System

Semba’s innovative Octave™ Chromatography System is an automated liquid chromatography instrument designed for purification of high-value chemical and biological compounds. This versatile bench top multicolumn continuous system is capable of simulated moving bed and other continuous chromatography protocols at fluid pressures up to 300 psi.

The Octave System carries eight column positions arranged in series and connected through a proprietary pneumatic valve array. The programmable 72-valve array contains no moving parts, occupies only 3 μl per valve, and responds within 100 ms. Fluid flow is controlled by four independent pumps. The valve and pump configuration provides ultimate flexibility in programming chromatographic protocols via the SembaPro™ Software, from running individual columns to schemes employing multiple columns, including simulated moving bed chromatography (SMBC). All flow paths are made of metal-free biocompatible materials that are also compatible with organic solvents for chemical applications. The Octave System is an "open platform" permitting the use of a wide range of chromatography columns and solid phases.

Benefits of SMBC

The countercurrent flow created by SMBC enables extremely efficient utilization of stationary and mobile phases. In single-column systems, separation actually occurs in a small fraction of the column at any one time, with the rest of the column performing no function other than occupying solvent and broadening bands. With SMBC, a series of small columns is used instead of one large column. Typically 50-70% of the solid phase is engaged in the separation zone, while the rest of the column material is being prepared for the next cycle of purification. Moreover, SMBC provides essentially an “infinite” column bed length without the costs associated with obtaining, operating, and maintaining large single columns.

• Higher recovery and purity
• Lower solvent consumption
• Higher productivity - up to 20-fold vs. batch systems
• Larger stationary phase particles, lower pressures
• Scalable from milligrams to tons of purified product
• Continuous process

Applications Bulk separations End product purifications Impurity analysis Chiral separation Process development

Targets mAbs, recombinant proteins Natural products Sugars, sugar alcohols, oligosaccharides Amino acids Organic acids Fatty acids Enantiomers Oligonucleotides