Introducing the Semba Octave^™
Chromatography System

The Octave Chromatography System

The smallest, most affordable simulated moving bed chromatography system in the world Countercurrent process improves resolution, productivity Continuous unattended purification, milligrams to grams Compatible with chemical and biological samples

The Semba Octave™ Chromatography System is an automated liquid chromatography instrument designed for purification of high-value chemical and biological compounds. This versatile bench top multicolumn system is capable of simulated moving bed protocols at fluid pressures up to 300 psi. The system carries eight column positions arranged in series and connected through a proprietary pneumatic valve system.

Fluid flow is controlled by four independent pumps, each of which is capable of flow rates from 0.05 to 10.00 ml/min with a precision of 0.5%. Each column is accessed by five inlet streams; four arising from the external inlets and one from the upstream column. Outlet flow from each column can be directed to any of four outlet ports as well as to the next column in the series. The valve configuration provides ultimate flexibility in programming chromatographic protocols via the SembaPro™ software, from running individual columns to schemes employing multiple columns, including simulated moving bed chromatography (SMBC).

The system easily accommodates continuous operation for multiple cycles of purification. All flow paths are made of metal-free biocompatible materials that are also compatible with organic solvents for chemical applications. The instrument brings the productivity of SMBC to the bench top.

Benefits of SMBC

The countercurrent flow created by SMBC enables extremely efficient utilization of stationary and mobile phases. In single- column systems, separation actually occurs in a small fraction of the column at any one time, with the rest of the column performing no function other than occupying solvent and broadening bands. With SMBC, a series of small columns is used instead of one large column. Typically 50-70% of the solid phase is engaged in the separation zone, while the rest of the column material is being prepared for the next cycle of purification. Moreover, SMBC provides essentially an “infinite” column bed length without the costs associated with obtaining, operating, and maintaining large single columns.

• Higher productivity - up to 20-fold vs. batch systems
• Higher recovery and purity
• Lower solvent consumption
• Larger stationary phase particles, lower pressures
• Scalable from milligrams to tons of purified product
• Ideal for chiral separations
• Continuous process
  

Applications

• Enantiomer separations using chiral stationary phases
• Recombinant protein purification using IMAC cartridges
• Antibody purification using Protein A/G or IEX cartridges
• Many others including affinity, SEC, HIC, ion exclusion