An important consideration for the type of column to be used with the Octave System is the expected back pressure that will be generated during operation. The total fluid back pressure limit for the instrument is 270 psi (18.6 bar, 1.86 MPa), which is due to the pressure limit of the pneumatic valve system (300 psi). Note that back pressures are additive. For example, if a separation has 3 columns in a given zone, and if the back pressure generated by a single column with the relevant solvent and zone flow rate is less than 90 psi, the column should be compatible with the system.
The increased productivity of SMBC allows the use of smaller columns with larger particle size and dramatically decreased plate numbers versus equivalent separations performed by HPLC. These conditions generate lower back pressures and enable higher flow rates, which is a key to the high productivity of SMBC. In general, columns having a particle size of 15 microns or greater (ideally >30 microns) are suitable for efficient performance on the Octave 10 System; particles >50 microns in diameter are recommended for the Octave 100 and 300 systems. Columns having a particle size less than 10 microns in diameter usually generate back pressures exceeding 270 psi under SMBC conditions and are not recommended for use with the Octave System.
Octave System components are available with different flow path materials to accommodate a variety of solvents. Pumps can be ordered with either stainless steel or PEEK heads and tubing. The standard Octave Chromatography Module is compatible with most aqueous and organic solvents including alcohols, aliphatic hydrocarbons, and many acids and bases. The Octave HS Chromatography Module is recommended for use with harsh solvents including partially halogenated and aromatic hydrocarbons, acetonitrile, esters, and ethers. Please contact Semba Biosciences for information on specific solvents.
Chiral Column Sets
The solid phases in CHIROBIOTIC™* columns are based on covalently bonding macrocyclic glycopeptides to a high purity 15 micron silica in such a way as to establish its stability while retaining essential components for chiral recognition.
The Astec CHIROBIOTIC columns for SMBC feature 5 cm x 10 mm I.D column dimensions for lower back pressure and higher flow rate, making them ideal for use on the Octave™ Chromatography System. Use the corresponding 25 cm x 4.6 mm I.D. column for method development.
* Manufactured by and trademark of Supelco/Sigma-Aldrich
- Rugged and reproducible for long term, reliable operation
- Selectors with 18 or 23 chiral centers
- Enantioselective for a wide range of chiral compounds
- Flexible mobile phases for optimum sample solubility and operation without memory effects
- CSPs offer mobile phase flexibility and are stable from 0-100% organic modifier.
CHIROBIOTIC V2 is based on bonding Vancomycin, which contains 18 chiral centers surrounding three pockets or cavities. Five aromatic ring structures bridge these strategic cavities. Hydrogen donor acceptor sites are readily available close to the ring structures.
CHIROBIOTIC T is based on bonding the amphoteric glycopeptide, Teicoplanin, which contains 23 chiral centers surrounding four pockets or cavities.
Sample: 5-methyl 5-phenylhydantoin
Mobile Phase: 100% MeOH
Column: CHIROBIOTIC T, 5 cm x 10 mm ID, 15 mm
Productivity: 70 mg/h each enantiomer
Total CSP: 25 g (8 columns)
Ion Exchange & Ion Exclusion Column Sets
Fructose-glucose separation using ion-exchange resins and SMBC is one of the most well-established industrial-scale SMBC applications. Relative to other separation methods, such as distillation, crystallization, and solvent extraction, ion exchange chromatography generally is faster, requires less energy, and produces less waste. The Octave™ System is the ideal tool for process research on separation of the monosaccharides from biomass, as well as for other fine chemical separations which require high-productivity and low-cost continuous ion exchange/ion exclusion chromatography processes. Semba Biosciences offers pack-to-order method development columns and SMB column sets for such applications as desalting, carbohydrate, oligosaccharide, polyol, and organic acid separations. Examples of such media include but are not limited to Dowex™ Monosphere™ 99Ca/320, Dowex 50WX8 Sodium Form, Dowex 50WX8 Sodium Form (USP L54 packing), Dowex 50WX8 Hydrogen Form (USP L17 packing), Dowex 50WX4 Sodium Form.Request a Quote
Reversed & Normal Phase Column Sets
Semba Biosciences offers pack-to-order method development columns and SMB column sets for a broad range of separations that use various customer-preferred normal and reversed phase chromatography media. Recommended particle sizes include 20, 30, and 50 micron. Please inquire for pricing and delivery time.Request a Quote