Semba Biosciences offers the following convenient reagents for protein sample preparation.
SembaSonic™ 10X Protein Extraction Reagent, a mild detergent-based solution for efficient and gentle protein extraction without harsh mechanical, chemical, and/or enzymatic treatments. Lyse-Aid™ Recombinant Lysozyme facilitates bacterial cell lysis alone or in combination with SembaSonic 10X Protein Extraction Reagent. The popular Benzonase Nuclease is available for effective removal of nucleic acids from protein preparations. Sonicase™ Non-detergent Protein Extraction Reagent is formulated to address the need for efficient extraction of proteins for structural studies and for other detergent-prohibitive applications. SembaChrom™ Imidazole Solution is a specially-treated concentrate with low UV absorbance which facilitates detection of recombinant proteins purified using immobilized metal affinity chromatography (IMAC).
SembaSonic™ Protein Extraction Reagent
A mild, efficient, detergent-based protein extraction reagent for reliable preparation of soluble protein fractions
SembaSonic 10X Protein Extraction Reagent is a proprietary concentrate containing a non-ionic detergent designed for preparation of cell lysates from E. coli. When used at a 1X final concentration, soluble proteins are gently released without denaturation or interference with activity. When combined with a buffer such as Tris or phosphate, Lyse-Aid™ Recombinant Lysozyme, and Benzonase® Nuclease, bacterial cell walls are effectively disrupted and nucleic acids are degraded to produce a non-viscous protein extract suitable for assays or chromatography. SembaSonic provides an easy, fast and economical substitute for physical disruption such as sonication or French Press homogenization. SembaSonic is ideal for rapid preparation of bacterial cell lysates for purification of recombinant proteins with Semba’s Octave™ Chromatography System. Use 5 ml 1X SembaSonic in a desired buffer per each gram of cell paste.
Sonicase™ Non-detergent Protein Extraction Reagent
Formulated for detergent-free, rapid, and efficient extraction of bacterial proteins
Dramatically reduce the viscosity of protein lysates, with a rapid, enzymatic treatment
Unit definition: One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml).
Lyse-Aid Recombinant Lysozyme
A great tool for enzymatic lysis of the E. coli cell walls
Unit definition: One unit of Lyse-Aid Recombinant Lysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A450 unit per minute at 25ºC in a 1-ml suspension (1 mg/ml) of BL21 cells in 0.5X SembaSonic Reagent diluted with 50 mM Tris-Cl, pH 7.5.
Imidazole, SembaChrom™ Grade, 2.5 M Solution
The convenience and assurance of pure, low UV-absorbing, qualified & pH adjusted reagent for IMAC protein purification