Imidazole, SembaChrom™ Grade, 2.5 M Solution
Imidazole, SembaChrom™ Grade, is a special preparation of imidazole having low UV absorbance. Solutions containing imidazole are commonly used for the purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Many commercial imidazole preparations contain UV-absorbing contaminants that interfere with spectrophotometric readings of protein solutions at 280 nm. SembaChrom Grade Imidazole has been processed to remove these contaminants, resulting in negligible background levels. The ready-to-use 2.5 M solution has been adjusted to pH 8.0 and filtered for convenient preparation of IMAC buffers.