The calculator is intended for the determination of initial parameters for SMBC separation in isocratic, 3-2-3 mode. for more information, please see section 7.6 of the Octave User Manual.

1. After inputting the initial separation values for a single column separation, along with column volume, flow rate, extra column dead volume (refer to section 7.6.4.3 of the Octave User Manual for more information) and the retention times for the two peaks from a binary separation, the calculator will determine the Henry constants and selectivity for your conditions.

2. In scenario 1 of the lower worksheet enter test values for the Feed, Desorbent, and Extract flow rates, and Switch time. The tool calculates Raffinate flow rate, zone flow rates (based on a 3-zone configuration), and m2 and m3. By adjusting the test values, values for m2 and m3 can be obtained that lie between the Henry constants of the two analytes. All values are replicated in scenarios 2-4.

3. Once conditions are identified within the desirable range (the values will turn green), scenarios 2 through 4 can be used to evaluate adjustment and optimization of the parameters established in scenario one.

4. Want to save your calculated conditions? Input your email address and the results will appear in your inbox. Addresses are strictly private, and never retained by Semba.